mouse anti arid2 Search Results


93
Novus Biologicals nbp2 43567
Nbp2 43567, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mouse anti arid2
Mouse Anti Arid2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc anti arid2 d8d8u
Anti Arid2 D8d8u, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology arid2
Figure 5. With PBRM1 deficiency, <t>ARID2</t> is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.
Arid2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/arid2/product/Santa Cruz Biotechnology
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Bethyl a302 230a
Figure 5. With PBRM1 deficiency, <t>ARID2</t> is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.
A302 230a, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc mouse monoclonal anti arid2 abcam ab51019 wb
Figure 5. With PBRM1 deficiency, <t>ARID2</t> is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.
Mouse Monoclonal Anti Arid2 Abcam Ab51019 Wb, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology resource source identifier mouse monoclonal anti-arid2 (e-3) santa cruz sc-166117
Figure 5. With PBRM1 deficiency, <t>ARID2</t> is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.
Resource Source Identifier Mouse Monoclonal Anti Arid2 (E 3) Santa Cruz Sc 166117, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GeneTex human arid2 gtx129444 antibody
Figure 5. With PBRM1 deficiency, <t>ARID2</t> is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.
Human Arid2 Gtx129444 Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc anti arid2
Figure 5. With PBRM1 deficiency, <t>ARID2</t> is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.
Anti Arid2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LI-COR donkeyagoat irdye 680rd
Figure 5. With PBRM1 deficiency, <t>ARID2</t> is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.
Donkeyagoat Irdye 680rd, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abiocode Inc rabbit anti-arid2
The translation of FMRP targets is limited by initiation rather than elongation rates. a) Ribosome footprints of FMRP targets Poe , osa , and HUWE1 in control and Fmr1 RNAi oocytes. b) Cumulative distribution plots showing nearly identical distributions of ribosome footprints for FMRP targets in wild-type and Fmr1 RNAi oocytes. c) Ribosome footprints of mouse FMRP targets <t>Arid2</t> , HUWE1 , and Auts2 . f) Cumulative distribution plot showing nearly identical distributions of ribosome footprints of Arid2 , HUWE1 , and Auts2 in wild-type vs FMRP-deficient tissue. e, f) Plots showing that all mouse FMRP targets as analyzed as in (d) have low K–S scores <0.3.
Rabbit Anti Arid2, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. With PBRM1 deficiency, ARID2 is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.

Journal: Molecular Cancer Research

Article Title: Loss of PBRM1 Alters Promoter Histone Modifications and Activates ALDH1A1 to Drive Renal Cell Carcinoma

doi: 10.1158/1541-7786.mcr-21-1039

Figure Lengend Snippet: Figure 5. With PBRM1 deficiency, ARID2 is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.

Article Snippet: The IP stepwas performed overnight at 4°C using 3 mg of targeting antibody (BRG1 (G-7; Santa Cruz Biotechnology, sc-17796, RRID:AB_626762) or ARID2 (E-3; Santa Cruz Biotechnology, sc-166117, RRID:AB_2060382) or mouse IgG (Santa Cruz Biotechnology, #sc-2025, RRID: AB_737182) prebound to 30-mL Protein G Dynabeads.

Techniques: Western Blot, Immunoprecipitation, Control, Fractionation, Comparison

Figure 6. ARID2 is required for increased ALDH1A1 expression and tumorsphere formation, whereas BRG1 and SNF5 are dispensable. A, C, and D, Western blot analysis exploring effects of knocking down (A) ARID2, (C) SNF5, and (D) BRG1 in 786-O control and PBRM1 shRNA #1 cells. A non-targeting siRNA was used as a control (labeled “C”). B and E, Tumorsphere assays in 786-O con- trol and PBRM1 shRNA #2 cells. Cells were transfected with non-targeting siRNA (labeled “C”) or targeting siRNAs 24 hours before plating for the tumorsphere assay. n ¼ 3–6/condition, from independent experiments; statistical testing was per- formed using an ordinary two-way ANOVA with Tukey’s multiple comparisons test between conditions within cell lines.

Journal: Molecular Cancer Research

Article Title: Loss of PBRM1 Alters Promoter Histone Modifications and Activates ALDH1A1 to Drive Renal Cell Carcinoma

doi: 10.1158/1541-7786.mcr-21-1039

Figure Lengend Snippet: Figure 6. ARID2 is required for increased ALDH1A1 expression and tumorsphere formation, whereas BRG1 and SNF5 are dispensable. A, C, and D, Western blot analysis exploring effects of knocking down (A) ARID2, (C) SNF5, and (D) BRG1 in 786-O control and PBRM1 shRNA #1 cells. A non-targeting siRNA was used as a control (labeled “C”). B and E, Tumorsphere assays in 786-O con- trol and PBRM1 shRNA #2 cells. Cells were transfected with non-targeting siRNA (labeled “C”) or targeting siRNAs 24 hours before plating for the tumorsphere assay. n ¼ 3–6/condition, from independent experiments; statistical testing was per- formed using an ordinary two-way ANOVA with Tukey’s multiple comparisons test between conditions within cell lines.

Article Snippet: The IP stepwas performed overnight at 4°C using 3 mg of targeting antibody (BRG1 (G-7; Santa Cruz Biotechnology, sc-17796, RRID:AB_626762) or ARID2 (E-3; Santa Cruz Biotechnology, sc-166117, RRID:AB_2060382) or mouse IgG (Santa Cruz Biotechnology, #sc-2025, RRID: AB_737182) prebound to 30-mL Protein G Dynabeads.

Techniques: Expressing, Western Blot, Control, shRNA, Labeling, Transfection

The translation of FMRP targets is limited by initiation rather than elongation rates. a) Ribosome footprints of FMRP targets Poe , osa , and HUWE1 in control and Fmr1 RNAi oocytes. b) Cumulative distribution plots showing nearly identical distributions of ribosome footprints for FMRP targets in wild-type and Fmr1 RNAi oocytes. c) Ribosome footprints of mouse FMRP targets Arid2 , HUWE1 , and Auts2 . f) Cumulative distribution plot showing nearly identical distributions of ribosome footprints of Arid2 , HUWE1 , and Auts2 in wild-type vs FMRP-deficient tissue. e, f) Plots showing that all mouse FMRP targets as analyzed as in (d) have low K–S scores <0.3.

Journal: Genetics

Article Title: FMRP-dependent production of large dosage-sensitive proteins is highly conserved

doi: 10.1093/genetics/iyac094

Figure Lengend Snippet: The translation of FMRP targets is limited by initiation rather than elongation rates. a) Ribosome footprints of FMRP targets Poe , osa , and HUWE1 in control and Fmr1 RNAi oocytes. b) Cumulative distribution plots showing nearly identical distributions of ribosome footprints for FMRP targets in wild-type and Fmr1 RNAi oocytes. c) Ribosome footprints of mouse FMRP targets Arid2 , HUWE1 , and Auts2 . f) Cumulative distribution plot showing nearly identical distributions of ribosome footprints of Arid2 , HUWE1 , and Auts2 in wild-type vs FMRP-deficient tissue. e, f) Plots showing that all mouse FMRP targets as analyzed as in (d) have low K–S scores <0.3.

Article Snippet: Rabbit anti-FMRP (1:50; Cell Signaling 4317), rabbit anti-Auts2 (1:50; Abcam ab96326), rabbit anti-Med13L (1:50; Bethyl Laboratories A302-420A), rabbit anti-HUWE1 (1:100; Thermo Fisher Scientific A300-486A), rabbit anti-Arid2 (1:50; Abiocode R2380-1), mouse anti-Arid1a (1:50; Santa Cruz sc-32761), rabbit anti-Fat1 (1:50; Abcam ab241372), rabbit anti-Ubr4 antibody (1:100; Abcam ab86738), and rabbit anti-Sptbn2 (1:50; Proteinteck 55107-1-AP) antibodies were used.

Techniques:

SFARI Class I and Class II ASD-relevant genes are translationally reduced in the Fmr1 KO mouse cortex.

Journal: Genetics

Article Title: FMRP-dependent production of large dosage-sensitive proteins is highly conserved

doi: 10.1093/genetics/iyac094

Figure Lengend Snippet: SFARI Class I and Class II ASD-relevant genes are translationally reduced in the Fmr1 KO mouse cortex.

Article Snippet: Rabbit anti-FMRP (1:50; Cell Signaling 4317), rabbit anti-Auts2 (1:50; Abcam ab96326), rabbit anti-Med13L (1:50; Bethyl Laboratories A302-420A), rabbit anti-HUWE1 (1:100; Thermo Fisher Scientific A300-486A), rabbit anti-Arid2 (1:50; Abiocode R2380-1), mouse anti-Arid1a (1:50; Santa Cruz sc-32761), rabbit anti-Fat1 (1:50; Abcam ab241372), rabbit anti-Ubr4 antibody (1:100; Abcam ab86738), and rabbit anti-Sptbn2 (1:50; Proteinteck 55107-1-AP) antibodies were used.

Techniques: