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Bethyl
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Santa Cruz Biotechnology
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GeneTex
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Cell Signaling Technology Inc
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LI-COR
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Abiocode Inc
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Image Search Results
Journal: Molecular Cancer Research
Article Title: Loss of PBRM1 Alters Promoter Histone Modifications and Activates ALDH1A1 to Drive Renal Cell Carcinoma
doi: 10.1158/1541-7786.mcr-21-1039
Figure Lengend Snippet: Figure 5. With PBRM1 deficiency, ARID2 is more highly expressed and remains bound to other SWI/SNF subunits. Western blot analysis of the indicated PBAF complex subunits in (A) 786-O cells and (B) A-704 cells. Immunoprecipitation (IP) experiments in 786-O cells for (C) BRG1 and (D) ARID2. Matched isotype IgG was used for control IPs. Inputs are aliquots taken from pre-cleared nuclear extracts before the IPs were performed. E, Western blot analysis of heavy fractions (#1–10, out of 24 total) from glycerol gradient fractionation of nuclear extracts from 786-O cells. The PRC2 protein EZH2 is shown for comparison.
Article Snippet: The IP stepwas performed overnight at 4°C using 3 mg of targeting antibody (BRG1 (G-7; Santa Cruz Biotechnology, sc-17796, RRID:AB_626762) or
Techniques: Western Blot, Immunoprecipitation, Control, Fractionation, Comparison
Journal: Molecular Cancer Research
Article Title: Loss of PBRM1 Alters Promoter Histone Modifications and Activates ALDH1A1 to Drive Renal Cell Carcinoma
doi: 10.1158/1541-7786.mcr-21-1039
Figure Lengend Snippet: Figure 6. ARID2 is required for increased ALDH1A1 expression and tumorsphere formation, whereas BRG1 and SNF5 are dispensable. A, C, and D, Western blot analysis exploring effects of knocking down (A) ARID2, (C) SNF5, and (D) BRG1 in 786-O control and PBRM1 shRNA #1 cells. A non-targeting siRNA was used as a control (labeled “C”). B and E, Tumorsphere assays in 786-O con- trol and PBRM1 shRNA #2 cells. Cells were transfected with non-targeting siRNA (labeled “C”) or targeting siRNAs 24 hours before plating for the tumorsphere assay. n ¼ 3–6/condition, from independent experiments; statistical testing was per- formed using an ordinary two-way ANOVA with Tukey’s multiple comparisons test between conditions within cell lines.
Article Snippet: The IP stepwas performed overnight at 4°C using 3 mg of targeting antibody (BRG1 (G-7; Santa Cruz Biotechnology, sc-17796, RRID:AB_626762) or
Techniques: Expressing, Western Blot, Control, shRNA, Labeling, Transfection
Journal: Genetics
Article Title: FMRP-dependent production of large dosage-sensitive proteins is highly conserved
doi: 10.1093/genetics/iyac094
Figure Lengend Snippet: The translation of FMRP targets is limited by initiation rather than elongation rates. a) Ribosome footprints of FMRP targets Poe , osa , and HUWE1 in control and Fmr1 RNAi oocytes. b) Cumulative distribution plots showing nearly identical distributions of ribosome footprints for FMRP targets in wild-type and Fmr1 RNAi oocytes. c) Ribosome footprints of mouse FMRP targets Arid2 , HUWE1 , and Auts2 . f) Cumulative distribution plot showing nearly identical distributions of ribosome footprints of Arid2 , HUWE1 , and Auts2 in wild-type vs FMRP-deficient tissue. e, f) Plots showing that all mouse FMRP targets as analyzed as in (d) have low K–S scores <0.3.
Article Snippet: Rabbit anti-FMRP (1:50; Cell Signaling 4317), rabbit anti-Auts2 (1:50; Abcam ab96326), rabbit anti-Med13L (1:50; Bethyl Laboratories A302-420A), rabbit anti-HUWE1 (1:100; Thermo Fisher Scientific A300-486A),
Techniques:
Journal: Genetics
Article Title: FMRP-dependent production of large dosage-sensitive proteins is highly conserved
doi: 10.1093/genetics/iyac094
Figure Lengend Snippet: SFARI Class I and Class II ASD-relevant genes are translationally reduced in the Fmr1 KO mouse cortex.
Article Snippet: Rabbit anti-FMRP (1:50; Cell Signaling 4317), rabbit anti-Auts2 (1:50; Abcam ab96326), rabbit anti-Med13L (1:50; Bethyl Laboratories A302-420A), rabbit anti-HUWE1 (1:100; Thermo Fisher Scientific A300-486A),
Techniques: